The embryonic shield appeared in 15h , and the fertilized egg entered the middle gastrula stage
2.
And it was found that in the embryonic shield area where convergence movements happening most extensively , cells " cortex were most highly stained
3.
Embryonic shield and its neighboring areas were especially highly stained and cortactin marked the cells undertaking active convergencing movement . the stained areas were belt - like along the anterior and posterior axis , firstly short and wide , with the progression of gastrulating , the stained belt was extending interiorly and narrowed , but the anterior and posterior terminals were sustainedly highly stained
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